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DPCR1 Polyclonal antibody

DPCR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31420-1-AP

Print datasheet

Synonyms

PBLT, MUCL3, C6orf37

验证数据展示

  • WB analysis using 31420-1-AP

    WB analysis using 31420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31420-1-AP (DPCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31420-1-AP

    IHC staining of human stomach cancer using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 31420-1-AP

    IHC staining of mouse stomach using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBGC-823 cells, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31420-1-AP targets DPCR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DPCR1 fusion protein Ag33318 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diffuse panbronchiolitis critical region 1
Synonyms PBLT, MUCL3, C6orf37
Calculated Molecular Weight56 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberNM_080870
Gene Symbol DPCR1
Gene ID (NCBI) 135656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ3MIW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diffuse panbronchiolitis critical region 1 (DPCR1) is located in the major histocompatibility complex (MHC) class I. DPCR1 is downregulated in invasive pituitary adenoma compared with that in non-invasive tumors, but upregulated in the precursor of gastric carcinogenesis (PMID: 29242154).

实验方案

Product Specific Protocols
WB protocol for DPCR1 antibody 31420-1-APDownload protocol
IHC protocol for DPCR1 antibody 31420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols