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验证数据展示

  • WB analysis using 21769-1-AP

    WB analysis using 21769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21769-1-AP (DPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21769-1-AP

    IF Staining of MCF-7 using 21769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DPF1 antibody (21769-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

DPF1 Polyclonal antibody

DPF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21769-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Zinc finger protein neuro-d4, SMARCG1, neuro d4, NEUD4, BRG1-associated factor 45B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, mouse cerebellum tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy DPF1 Ready-To-Use IHC Kit. DPF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21769-1-AP targets DPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 DPF1 fusion protein Ag16409 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 D4, zinc and double PHD fingers family 1
别名 Zinc finger protein neuro-d4, SMARCG1, neuro d4, NEUD4, BRG1-associated factor 45B
计算分子量 414 aa, 47 kDa
观测分子量38-47 kDa
GenBank蛋白编号BC125153
基因名称 DPF1
Gene ID (NCBI) 8193
RRIDAB_3085672
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92782
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPF1 antibody 21769-1-APDownload protocol
IF protocol for DPF1 antibody 21769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols