验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, Y79 cells, HepG2 cells, mouse thymus tissue, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human testis tissue, human pituitary adenoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Planning an IHC experiment? We recommend our IHCeasy DPF2 Ready-To-Use IHC Kit. DPF2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12111-1-AP targets DPF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DPF2 fusion protein Ag2756 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | D4, zinc and double PHD fingers family 2 |
| Synonyms | ubi d4, REQ, Protein requiem, BRG1-associated factor 45D, BRG1 associated factor 45D |
| Calculated Molecular Weight | 391 aa, 44 kDa |
| Observed Molecular Weight | 44-50 kDa |
| GenBank Accession Number | BC014889 |
| Gene Symbol | DPF2 |
| Gene ID (NCBI) | 5977 |
| RRID | AB_2095574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92785 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPF2 antibody 12111-1-AP | Download protocol |
| IHC protocol for DPF2 antibody 12111-1-AP | Download protocol |
| IF protocol for DPF2 antibody 12111-1-AP | Download protocol |
| IP protocol for DPF2 antibody 12111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Anal Chem Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling. | ||
J Cancer DPF2 overexpression correlates with immune infiltration and dismal prognosis in hepatocellular carcinoma | ||
Front Neurosci BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.
| ||
Front Neuroanat The BAF45D Protein Is Preferentially Expressed in Adult Neurogenic Zones and in Neurons and May Be Required for Retinoid Acid Induced PAX6 Expression.
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Biosci Biotechnol Biochem BAF45D knockdown decreases cell viability, inhibits colony formation, induces cell apoptosis and S-phase arrest in human pancreatic cancer cells.
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