验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, Y79 cells, HepG2 cells, mouse thymus tissue, K-562 cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lung cancer tissue, human testis tissue, human pituitary adenoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12111-1-AP targets DPF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | DPF2 fusion protein Ag2756 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | D4, zinc and double PHD fingers family 2 |
别名 | ubi d4, REQ, Protein requiem, BRG1-associated factor 45D, BRG1 associated factor 45D |
计算分子量 | 391 aa, 44 kDa |
观测分子量 | 44-50 kDa |
GenBank蛋白编号 | BC014889 |
基因名称 | DPF2 |
Gene ID (NCBI) | 5977 |
RRID | AB_2095574 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92785 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells.
实验方案
Product Specific Protocols | |
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WB protocol for DPF2 antibody 12111-1-AP | Download protocol |
IHC protocol for DPF2 antibody 12111-1-AP | Download protocol |
IF protocol for DPF2 antibody 12111-1-AP | Download protocol |
IP protocol for DPF2 antibody 12111-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Anal Chem Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling. | ||
J Cancer DPF2 overexpression correlates with immune infiltration and dismal prognosis in hepatocellular carcinoma | ||
Front Neurosci BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.
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Front Neuroanat The BAF45D Protein Is Preferentially Expressed in Adult Neurogenic Zones and in Neurons and May Be Required for Retinoid Acid Induced PAX6 Expression.
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Biosci Biotechnol Biochem BAF45D knockdown decreases cell viability, inhibits colony formation, induces cell apoptosis and S-phase arrest in human pancreatic cancer cells.
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