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DPH2 Polyclonal antibody

DPH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14866-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 14866-1-AP

    WB analysis of HeLa using 14866-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14866-1-AP (DPH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14866-1-AP targets DPH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DPH2 fusion protein Ag6678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DPH2 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001389
Gene Symbol DPH2
Gene ID (NCBI) 1802
RRIDAB_2093254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

实验方案

Product Specific Protocols
WB protocol for DPH2 antibody 14866-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols