验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DPM1 Ready-To-Use IHC Kit. DPM1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12403-2-AP targets DPM1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | DPM1 fusion protein Ag1128 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit |
| 别名 | DPM synthase subunit 1, DPM synthase, Dolichyl-phosphate beta-D-mannosyltransferase subunit 1, Dolichol-phosphate mannosyltransferase subunit 1, Dolichol-phosphate mannose synthase subunit 1 |
| 计算分子量 | 260 aa, 30 kDa |
| 观测分子量 | 27-32 kDa |
| GenBank蛋白编号 | BC007073 |
| 基因名称 | DPM1 |
| Gene ID (NCBI) | 8813 |
| RRID | AB_2093270 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60762 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPM1 antibody 12403-2-AP | Download protocol |
| IHC protocol for DPM1 antibody 12403-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol DPM1 modulates desmosomal adhesion and epidermal differentiation through SERPINB5
| ||
Front Genet Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm. | ||
FEBS Lett Aspirin damages the cell wall of Saccharomyces cerevisiae by inhibiting the expression and activity of dolichol-phosphate mannose synthase 1. |
