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DPP6 Polyclonal antibody

DPP6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28143-1-AP

Print datasheet

Synonyms

DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein

验证数据展示

  • WB analysis using 28143-1-AP

    WB analysis using 28143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28143-1-AP (DPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 28143-1-AP

    IP experiment of mouse brain using 28143-1-AP

    IP result of anti-DPP6 (IP:28143-1-AP, 4ug; Detection:28143-1-AP 1:4000) with mouse brain tissue lysate 1840 ug.

  • IF Staining of SH-SY5Y using 28143-1-AP

    IF Staining of SH-SY5Y using 28143-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DPP6 antibody (28143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28143-1-AP targets DPP6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DPP6 fusion protein Ag27338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dipeptidyl-peptidase 6
Synonyms DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein
Observed Molecular Weight 98-101 kDa
GenBank Accession NumberBC150304
Gene Symbol DPP6
Gene ID (NCBI) 1804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42658
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP6 (Dipeptidyl aminopeptidase-like protein 6) is also named as DPPX and Dipeptidyl peptidase VI (DPP VI). DPP6 is a type II transmembrane protein, and DPP6 is a multifunctional protein with considerable impact on brain development and function (PMID: 35395074). In breast cancer tissues, DPP6 has low expression at the transcription and protein levels, and DPP6 may serve as a tumour suppressor in tumour development (PMID: 36531033).

实验方案

Product Specific Protocols
WB protocol for DPP6 antibody 28143-1-APDownload protocol
IF protocol for DPP6 antibody 28143-1-APDownload protocol
IP protocol for DPP6 antibody 28143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols