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DPP7 Polyclonal antibody

DPP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19018-1-AP

Print datasheet

Synonyms

DPP2, DPP II, Dipeptidyl peptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase 2

验证数据展示

  • WB analysis using 19018-1-AP

    WB analysis using 19018-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19018-1-AP (DPP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19018-1-AP

    IHC staining of human ovary cancer using 19018-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19018-1-AP (DPP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU-937 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19018-1-AP targets DPP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DPP7 fusion protein Ag13556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dipeptidyl-peptidase 7
Synonyms DPP2, DPP II, Dipeptidyl peptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC016961
Gene Symbol DPP7
Gene ID (NCBI) 29952
RRIDAB_10641993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP7 (also known as DPP-II and quiescent cell proline dipeptidase, QPP), is the first reported protease that contains a leucine zipper motif through which the functional homodimer has been predicted to be formed (PMID: 22952628). DPP7 can be detected as 54 kDa (full length) and ~50 kDa (signal peptide cleaved).

实验方案

Product Specific Protocols
WB protocol for DPP7 antibody 19018-1-APDownload protocol
IHC protocol for DPP7 antibody 19018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines.

Authors - Alexander Schepsky
View Article