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验证数据展示

  • WB analysis using 19018-1-AP

    WB analysis using 19018-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19018-1-AP (DPP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19018-1-AP

    IHC staining of human ovary cancer using 19018-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19018-1-AP (DPP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DPP7 Polyclonal antibody

DPP7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19018-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DPP2, DPP II, Dipeptidyl peptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19018-1-AP targets DPP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 DPP7 fusion protein Ag13556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 7
别名 DPP2, DPP II, Dipeptidyl peptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase 2
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC016961
基因名称 DPP7
Gene ID (NCBI) 29952
RRIDAB_10641993
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP7 (also known as DPP-II and quiescent cell proline dipeptidase, QPP), is the first reported protease that contains a leucine zipper motif through which the functional homodimer has been predicted to be formed (PMID: 22952628). DPP7 can be detected as 54 kDa (full length) and ~50 kDa (signal peptide cleaved).

实验方案

Product Specific Protocols
WB protocol for DPP7 antibody 19018-1-APDownload protocol
IHC protocol for DPP7 antibody 19018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines.

Authors - Alexander Schepsky
View Article