• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DPP9 Polyclonal antibody

DPP9 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11568-1-AP

Print datasheet

Synonyms

dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2

验证数据展示

  • WB analysis of HepG2 using 11568-1-AP

    WB analysis of HepG2 using 11568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11568-1-AP

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 11568-1-AP

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11568-1-AP

    IF Staining of HepG2 using 11568-1-AP

    Immunofluorescent analysis of HepG2 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, MCF-7 cells, mouse heart tissue, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11568-1-AP targets DPP9 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DPP9 fusion protein Ag2145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dipeptidyl-peptidase 9
Synonyms dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2
Calculated Molecular Weight 863 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC037948
Gene Symbol DPP9
Gene ID (NCBI) 91039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dipeptidyl peptidase 9(DPP9), is a member of the S9B family in clan SC of the serine proteases. DPP9 has 3 isoforms with MW of 95, 98 and 101 kDa. DPP9 is involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.