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DPY30 Polyclonal antibody

DPY30 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16281-1-AP

Print datasheet

Synonyms

dpy 30 homolog (C. elegans), Dpy 30 like protein, Dpy 30L, DPY30, HDPY 30, Protein dpy 30 homolog, Saf19

验证数据展示

  • IHC staining of human stomach cancer using 16281-1-AP

    IHC staining of human stomach cancer using 16281-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 16281-1-AP

    IF Staining of A431 using 16281-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DPY30 antibody (16281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inhuman stomach cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16281-1-AP targets DPY30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DPY30 fusion protein Ag9370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dpy-30 homolog (C. elegans)
Synonyms dpy 30 homolog (C. elegans), Dpy 30 like protein, Dpy 30L, DPY30, HDPY 30, Protein dpy 30 homolog, Saf19
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC015970
Gene Symbol DPY30
Gene ID (NCBI) 84661
RRIDAB_2918044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C005
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DPY30 antibody 16281-1-APDownload protocol
IF protocol for DPY30 antibody 16281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Biol

A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance

Authors - Jiaojiao Hao
View Article