验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10525-1-AP targets CRMP5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CRMP5 fusion protein Ag0783 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dihydropyrimidinase-like 5 |
| Synonyms | DPYSL5, DPYSL 5, DPYS L5, Dihydropyrimidinase-related protein 5, CRMP-5 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC002874 |
| Gene Symbol | CRMP5 |
| Gene ID (NCBI) | 56896 |
| RRID | AB_2094644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BPU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPYSL5(Dihydropyrimidinase-related protein 5) is also named as CRMP5, ULIP6 and belongs to the hydantoinase/dihydropyrimidinase subfamily. It may have a function in neuronal differentiation and/or axon growth. SCLCs universally express CRMP5 and in thymoma, none of the CRMPs are detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5.The normal CRMP5 is probably not expressed by tumorous epithelial cells.(PMID:16519949).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IHC protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IF protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| IP protocol for CRMP5 antibody 10525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Int Med Res Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix. | ||
Neurobiol Dis The ataxia-telangiectasia disease protein ATM controls vesicular protein secretion via CHGA and microtubule dynamics via CRMP5 |