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DRD5 Polyclonal antibody

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 20310-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA and More (1)

dopamine receptor D5, Dopamine D5 receptor, DBDR, D1beta dopamine receptor, D(5) dopamine receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20310-1-AP targets DRD5 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 DRD5 fusion protein Ag14145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dopamine receptor D5
别名 dopamine receptor D5, Dopamine D5 receptor, DBDR, D1beta dopamine receptor, D(5) dopamine receptor
计算分子量 477 aa, 53 kDa
观测分子量 50-53 kDa
GenBank蛋白编号BC009748
基因名称 DRD5
Gene ID (NCBI) 1816
RRIDAB_10699880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21918
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

实验方案

Product Specific Protocols
WB protocol for DRD5 antibody 20310-1-APDownload protocol
IHC protocol for DRD5 antibody 20310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

PMID: 25594175

Authors - Yiqing Yan
  • KD Validated
mouseWB

Mol Cell

Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.

Authors - Yuqing Wu
  • KO Validated
mouseIHC

Int J Mol Sci

Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review.

Authors - Magdalena Kurnik-Łucka
humanWB

ACS Chem Neurosci

Development of a PC12 Cell Based Assay for Screening Catechol-O-methyltransferase Inhibitors

Authors - Gongliang Zhang
humanWB,IF

Cell Oncol (Dordr)

Activation of dopamine receptor D1 inhibits glioblastoma tumorigenicity by regulating autophagic activity.

Authors - Kang Yang
mouseWB

J Neurochem

Validation of the Targeted Metabolomic Pathway in the Hippocampus and Comparative Analysis with the Prefrontal Cortex of Social Defeat Model Mice.

Authors - Ke Xu
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