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DROSHA Polyclonal antibody

DROSHA Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27958-1-AP

Print datasheet

Synonyms

DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN

验证数据展示

  • WB analysis using 27958-1-AP

    WB analysis using 27958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 27958-1-AP

    IF Staining of U2OS using 27958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of U2OS using 27958-1-AP

    IF Staining of U2OS using 27958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, Hela cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27958-1-AP targets DROSHA in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen DROSHA fusion protein Ag27533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease type III, nuclear
Synonyms DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN
Calculated Molecular Weight 159 aa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_001100412
Gene Symbol DROSHA
Gene ID (NCBI) 29102
RRIDAB_2918137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.

实验方案

Product Specific Protocols
WB protocol for DROSHA antibody 27958-1-APDownload protocol
IF protocol for DROSHA antibody 27958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

N4-acetylcytidine modifies primary microRNAs for processing in cancer cells

Authors - Hailong Zhang
View Article