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  • Featured Product
  • KD/KO Validated

DRP1 (C-terminal) Recombinant antibody, PBS Only

DRP1 (C-terminal) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A19

Cat no : 81561-1-PBS

Print datasheet

Synonyms

DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1

验证数据展示

  • WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

    WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81561-1-RR (same clone as 81561-1-PBS)

    WB analysis of HEK-293 using 81561-1-RR (same clone as 81561-1-PBS)

    WB result of DRP1 (C-terminal) antibody (81561-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HEK-293 cells. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81561-1-RR (same clone as 81561-1-PBS)

    FC experiment of HeLa using 81561-1-RR (same clone as 81561-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (81561-1-RR, Clone:2A19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

    WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

    IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

    IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 81561-1-RR (same clone as 81561-1-PBS)

    IF Staining of NIH/3T3 using 81561-1-RR (same clone as 81561-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DRP1 (C-terminal) antibody (81561-1-RR, Clone: 2A19 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81561-1-RR

    Affinity and Kinetic Characterization of 81561-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81561-1-RR against Human DRP1 were performed. The affinity constant is below 1 pM.

产品信息

81561-1-PBS targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dynamin 1-like
Synonyms DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1
Calculated Molecular Weight 736 aa, 82 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC024590
Gene Symbol DRP1
Gene ID (NCBI) 10059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00429
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.