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  • Featured Product
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DSG2 Polyclonal antibody

DSG2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29206-1-AP

Print datasheet

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

验证数据展示

  • WB analysis using 29206-1-AP

    WB analysis using 29206-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 29206-1-AP (DSG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 29206-1-AP

    WB analysis of HeLa using 29206-1-AP

    WB result of DSG2 antibody (29206-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HeLa cells.

  • IF Staining of MCF-7 using 29206-1-AP

    IF Staining of MCF-7 using 29206-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DSG2 antibody (29206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy DSG2 Ready-To-Use IHC Kit. DSG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29206-1-AP targets DSG2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen DSG2 fusion protein Ag20633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmoglein 2
Synonyms ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC
Calculated Molecular Weight 1118 aa, 122 kDa
Observed Molecular Weight150-160 kDa
GenBank Accession NumberBC099655
Gene Symbol DSG2
Gene ID (NCBI) 1829
RRIDAB_2918248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DSG2 antibody 29206-1-APDownload protocol
IF protocol for DSG2 antibody 29206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols