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DSN1 Polyclonal antibody

DSN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17742-1-AP

Print datasheet

Synonyms

C20orf172, DSN1, MIS13

验证数据展示

  • WB analysis of Jurkat using 17742-1-AP

    WB analysis of Jurkat using 17742-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17742-1-AP (DSN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17742-1-AP

    IHC staining of human breast cancer using 17742-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 17742-1-AP

    IHC staining of human breast cancer using 17742-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17742-1-AP targets DSN1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DSN1 fusion protein Ag11988 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DSN1, MIND kinetochore complex component, homolog (S. cerevisiae)
Synonyms C20orf172, DSN1, MIS13
Calculated Molecular Weight 340 aa, 38 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC026011
Gene Symbol DSN1
Gene ID (NCBI) 79980
RRIDAB_2878431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H410
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DSN1, also named as C20orf172 and MIS13, is required to shape an interface between the Mis12 complex and Blinkin, the counterpart of Spc105. DSN1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. DSN1 has four isoforms with MW 27-33 kDa and 38-40 kDa.

实验方案

Product Specific Protocols
WB protocol for DSN1 antibody 17742-1-APDownload protocol
IHC protocol for DSN1 antibody 17742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.

Authors - Haining Zhou
View Article
humanWB

J Exp Clin Cancer Res

Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma.

Authors - Huakai Wang
View Article