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验证数据展示

  • IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    IF Staining of HeLa using 55396-1-AP (same clone as 55396-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

  • WB analysis using 55396-1-AP (same clone as 55396-1-PBS)

    WB analysis using 55396-1-AP (same clone as 55396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55396-1-PBS in a different storage buffer formulation.

DSPP Polyclonal antibody, PBS Only

DSPP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 55396-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

55396-1-PBS targets DSPP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dentin sialophosphoprotein
别名
计算分子量 131 kDa
观测分子量 130-140 kDa
GenBank蛋白编号NM_014208
基因名称 DSPP
Gene ID (NCBI) 1834
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZW4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).