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验证数据展示

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 28438-1-AP

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 28438-1-AP

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

DST Polyclonal antibody

DST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28438-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DMH, D6S1101, CATX 15, BPAG1, BPA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

28438-1-AP targets DST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DST fusion protein Ag28833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dystonin
Synonyms DMH, D6S1101, CATX 15, BPAG1, BPA
Calculated Molecular Weight 861 kDa
GenBank Accession NumberNM_001144769
Gene Symbol DST
Gene ID (NCBI) 667
RRIDAB_2881141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03001
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DST antibody 28438-1-APDownload protocol
IHC protocol for DST antibody 28438-1-APDownload protocol
IF protocol for DST antibody 28438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Paxbp1 is indispensable for the maintenance of epidermal homeostasis

Authors - Cong Huang
View Article