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验证数据展示

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 28438-1-AP

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 28438-1-AP

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

DST Polyclonal antibody

DST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28438-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DMH, D6S1101, CATX 15, BPAG1, BPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

28438-1-AP targets DST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 DST fusion protein Ag28833 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dystonin
别名 DMH, D6S1101, CATX 15, BPAG1, BPA
计算分子量 861 kDa
GenBank蛋白编号NM_001144769
基因名称 DST
Gene ID (NCBI) 667
RRIDAB_2881141
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03001
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DST antibody 28438-1-APDownload protocol
IHC protocol for DST antibody 28438-1-APDownload protocol
IF protocol for DST antibody 28438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Paxbp1 is indispensable for the maintenance of epidermal homeostasis

Authors - Cong Huang
View Article