验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, MCF-7 cells, PC-3 cells, SKOV-3 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10962-2-AP targets Destrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Destrin fusion protein Ag1402 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | destrin (actin depolymerizing factor) |
Synonyms | DSTN, DSN, ADF, Actin-depolymerizing factor, ACTDP |
Calculated Molecular Weight | 19 kDa |
Observed Molecular Weight | 18 kDa |
GenBank Accession Number | BC009477 |
Gene Symbol | Destrin |
Gene ID (NCBI) | 11034 |
RRID | AB_2230671 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P60981 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Destrin antibody 10962-2-AP | Download protocol |
IHC protocol for Destrin antibody 10962-2-AP | Download protocol |
IF protocol for Destrin antibody 10962-2-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |