验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, MCF-7 cells, PC-3 cells, SKOV-3 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10962-2-AP targets Destrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Destrin fusion protein Ag1402 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | destrin (actin depolymerizing factor) |
别名 | DSTN, DSN, ADF, Actin-depolymerizing factor, ACTDP |
计算分子量 | 19 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC009477 |
基因名称 | Destrin |
Gene ID (NCBI) | 11034 |
RRID | AB_2230671 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P60981 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.
实验方案
Product Specific Protocols | |
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WB protocol for Destrin antibody 10962-2-AP | Download protocol |
IHC protocol for Destrin antibody 10962-2-AP | Download protocol |
IF protocol for Destrin antibody 10962-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |