• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 12896-1-AP

    WB analysis of HeLa using 12896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12896-1-AP

    IP experiment of mouse testis using 12896-1-AP

    IP result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug.

  • IF Staining of HeLa using 12896-1-AP

    IF Staining of HeLa using 12896-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DTL Polyclonal antibody

DTL Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12896-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

L2DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12896-1-AP targets DTL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DTL fusion protein Ag3589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 denticleless homolog (Drosophila)
别名 L2DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1
计算分子量 730 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC033540
基因名称 DTL
Gene ID (NCBI) 51514
RRIDAB_10639514
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

实验方案

Product Specific Protocols
WB protocol for DTL antibody 12896-1-APDownload protocol
IF protocol for DTL antibody 12896-1-APDownload protocol
IP protocol for DTL antibody 12896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Adv

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.

Authors - Chunxia Zhang
View Article
humanWB

Proteomics

Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs

Authors - Chengli Yu
View Article