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DTL Recombinant antibody

DTL Recombinant Antibody for IF/ICC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230144C9

Cat No : 83107-1-RR

Print datasheet

Synonyms

CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP

验证数据展示

  • IF Staining of HeLa using 83107-1-RR

    IF Staining of HeLa using 83107-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DTL antibody (83107-1-RR, Clone: 230144C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83107-1-RR

    FC experiment of HeLa using 83107-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DTL Recombinant antibody (83107-1-RR, Clone:230144C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83107-1-RR targets DTL in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC, ELISA Application Description
Tested Reactivity Human
Immunogen DTL fusion protein Ag33391 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name denticleless homolog (Drosophila)
Synonyms CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP
Calculated Molecular Weight 730 aa, 79 kDa
GenBank Accession NumberBC033540
Gene Symbol DTL
Gene ID (NCBI) 51514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

实验方案

Product Specific Protocols
IF protocol for DTL antibody 83107-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols