验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83107-1-RR targets DTL in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | DTL fusion protein Ag33391 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | denticleless homolog (Drosophila) |
别名 | Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1, CDT2 |
计算分子量 | 730 aa, 79 kDa |
GenBank蛋白编号 | BC033540 |
基因名称 | DTL |
Gene ID (NCBI) | 51514 |
RRID | AB_3670819 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9NZJ0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).
实验方案
Product Specific Protocols | |
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IF protocol for DTL antibody 83107-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |