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DTNA Polyclonal antibody

DTNA Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10741-1-AP

Print datasheet

Synonyms

Alpha dystrobrevin, D18S892E, DRP3, DTN, DTN A, DTNA, Dystrobrevin alpha, dystrobrevin, alpha, Dystrophin related protein 3, LVNC1

验证数据展示

  • WB analysis using 10741-1-AP

    WB analysis using 10741-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10741-1-AP (DTNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10741-1-AP

    IP experiment of mouse brain using 10741-1-AP

    IP result of anti-DTNA (IP:10741-1-AP, 4ug; Detection:10741-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse heart tissue, rat heart
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10741-1-AP targets DTNA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DTNA fusion protein Ag1075 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dystrobrevin, alpha
Synonyms Alpha dystrobrevin, D18S892E, DRP3, DTN, DTN A, DTNA, Dystrobrevin alpha, dystrobrevin, alpha, Dystrophin related protein 3, LVNC1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight25, 49-59 kDa, 78-85 kDa
GenBank Accession NumberBC005300
Gene Symbol DTNA
Gene ID (NCBI) 1837
RRIDAB_2093895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DTNA (dystorbrevin alpha) is a cytoskeleton associated protein and an essential component of dystrophin protein complex. It is highly expressed in brain, heart and skeletal muscle. Alternative splicing generates several isoforms ranging from 22 to 84 kDa.

实验方案

Product Specific Protocols
WB protocol for DTNA antibody 10741-1-APDownload protocol
IP protocol for DTNA antibody 10741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols