验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human testis tissue, mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 1 publications below |
产品信息
11132-1-AP targets Dysbindin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Dysbindin fusion protein Ag1613 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dystrobrevin binding protein 1 |
| 别名 | DBND, DTNBP1, DTNBP1a, DTNBP1b, Dysbindin, Dysbindin 1, dystrobrevin binding protein 1, HPS7, HPS7 protein, My031, SDY |
| 计算分子量 | 351 aa, 39 kDa |
| 观测分子量 | 48 kDa, 36 kDa |
| GenBank蛋白编号 | BC011912 |
| 基因名称 | Dysbindin |
| Gene ID (NCBI) | 84062 |
| RRID | AB_11182503 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dysbindin antibody 11132-1-AP | Download protocol |
| IHC protocol for Dysbindin antibody 11132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Total Environ TRIM24-DTNBP1-ATP7A mediated astrocyte cuproptosis in cognition and memory dysfunction caused by Y2O3 NPs | ||
J Cell Biol BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. | ||
Am J Respir Cell Mol Biol Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes. | ||
Neuropharmacology Mice with dopaminergic neuron-specific deletion of DTNBP-1 gene show blunted nucleus accumbens dopamine release and associated behaviors. | ||
Neuroscience Protein kinase B/Akt1 phosphorylates dysbindin-1A at serine 10 to regulate neuronal development. | ||
Br J Pharmacol Dopaminergic signaling and behavioral alterations by Comt-Dtnbp1 genetic interaction and their clinical relevance |
