验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, A375 cells |
| Positive IHC detected in | human colon tissue, human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse olfactory epithelium tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF) | IF : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
18350-1-AP targets DTX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | DTX1 fusion protein Ag13172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deltex homolog 1 (Drosophila) |
| 别名 | Protein deltex-1, hDx 1, hDTX1, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX1 |
| 计算分子量 | 620 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC048216 |
| 基因名称 | DTX1 |
| Gene ID (NCBI) | 1840 |
| RRID | AB_2878536 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86Y01 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deltex homolog 1 (E3 ubiquitin-protein ligase DTX1) is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.DTX1 is conserved from Drosophila, and is expressed in a wide array of tissues, particularly in the central nervous system, testes, and endothelial cells, mainly colocalized with Notch1.It contains a RING-type zinc finger and 2 WWE domains.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DTX1 antibody 18350-1-AP | Download protocol |
| IHC protocol for DTX1 antibody 18350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virus Res Gambogic acid inhibits HBx-mediated hepatitis B virus replication by targeting the DTX1-Notch signaling pathway
| ||
Cancer Sci Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma. |
