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DULLARD Polyclonal antibody

DULLARD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22088-1-AP

Print datasheet

Synonyms

Serine/threonine-protein phosphatase dullard, NET56, HSA011916, EC:3.1.3.16, CTDNEP1

验证数据展示

  • WB analysis of PC-3 using 22088-1-AP

    WB analysis of PC-3 using 22088-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22088-1-AP (DULLARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22088-1-AP

    IHC staining of human ovary cancer using 22088-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22088-1-AP (DULLARD antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 22088-1-AP

    IF Staining of PC-3 using 22088-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using DULLARD antibody (22088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inPC-3 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22088-1-AP targets DULLARD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DULLARD fusion protein Ag17288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dullard homolog (Xenopus laevis)
Synonyms Serine/threonine-protein phosphatase dullard, NET56, HSA011916, EC:3.1.3.16, CTDNEP1
Calculated Molecular Weight 244 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC009295
Gene Symbol DULLARD
Gene ID (NCBI) 23399
RRIDAB_2878991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DULLARD antibody 22088-1-APDownload protocol
IHC protocol for DULLARD antibody 22088-1-APDownload protocol
IF protocol for DULLARD antibody 22088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols