验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25259-1-AP targets DUPD1 / DUSP27 in WB, ELISA applications and shows reactivity with human, mouse , rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse , rat |
| 免疫原 | DUPD1 / DUSP27 fusion protein Ag19658 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase and pro isomerase domain containing 1 |
| 别名 | DUPD1, DUSP27, FMDSP |
| 计算分子量 | 220 aa, 25 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC137322 |
| 基因名称 | DUSP27 |
| Gene ID (NCBI) | 338599 |
| RRID | AB_3085779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q68J44 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dupd1also named as Dusp27, has been categorized as an atypical dual-specificity phosphatase (Dusp) capable of dephosphorylating both tyrosine and serine/threonine residues. It's involved in the modulation of intracellular signaling cascades. In skeletal muscle Dupd1 regulates systemic glucose homeostasis by activating AMPK, which is an energy sensor protein kinase. Dupd1 also affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUPD1 / DUSP27 antibody 25259-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |