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DUPD1 / DUSP27 Polyclonal antibody

DUPD1 / DUSP27 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25259-1-AP

Print datasheet

Synonyms

DUPD1, DUSP27, FMDSP

验证数据展示

  • WB analysis using 25259-1-AP

    WB analysis using 25259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25259-1-AP (DUPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25259-1-AP targets DUPD1 / DUSP27 in WB, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse , rat
Immunogen DUPD1 / DUSP27 fusion protein Ag19658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase and pro isomerase domain containing 1
Synonyms DUPD1, DUSP27, FMDSP
Calculated Molecular Weight 220 aa, 25 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC137322
Gene Symbol DUSP27
Gene ID (NCBI) 338599
RRIDAB_3085779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68J44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dupd1also named as Dusp27, has been categorized as an atypical dual-specificity phosphatase (Dusp) capable of dephosphorylating both tyrosine and serine/threonine residues. It's involved in the modulation of intracellular signaling cascades. In skeletal muscle Dupd1 regulates systemic glucose homeostasis by activating AMPK, which is an energy sensor protein kinase. Dupd1 also affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy.

实验方案

Product Specific Protocols
WB protocol for DUPD1 / DUSP27 antibody 25259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols