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DUSP13 Polyclonal antibody

DUSP13 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10909-1-AP

Print datasheet

Synonyms

MDSP, EC:3.1.3.16, DUSP13B, DUSP13A, Dual specificity protein phosphatase 13B

验证数据展示

  • WB analysis using 10909-1-AP

    WB analysis using 10909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10909-1-AP (DUSP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 10909-1-AP

    IP experiment of mouse testis using 10909-1-AP

    IP result of anti-DUSP13 (IP:10909-1-AP, 4ug; Detection:10909-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • IHC staining of human colon cancer using 10909-1-AP

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10909-1-AP

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10909-1-AP

    IHC staining of human colon cancer using 10909-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10909-1-AP (DUSP13 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, human skeletal muscle tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10909-1-AP targets DUSP13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DUSP13 fusion protein Ag1348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 13
Synonyms MDSP, EC:3.1.3.16, DUSP13B, DUSP13A, Dual specificity protein phosphatase 13B
Calculated Molecular Weight 22 kDa, 36 kDa
Observed Molecular Weight 22 kDa, 36 kDa
GenBank Accession NumberBC009778
Gene Symbol DUSP13
Gene ID (NCBI) 51207
RRIDAB_2230734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UII6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP13 antibody 10909-1-APDownload protocol
IHC protocol for DUSP13 antibody 10909-1-APDownload protocol
IP protocol for DUSP13 antibody 10909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomedicines

The Strong Activation of p53 Tumor Suppressor Drives the Synthesis of the Enigmatic Isoform of DUSP13 Protein

Authors - Małgorzata Krześniak
View Article