验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells, COLO 320 cells, HeLa cells, mouse brain tissue |
Positive IHC detected in | human colon tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
14237-1-AP targets DUSP16 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | DUSP16 fusion protein Ag5479 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dual specificity phosphatase 16 |
别名 | DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7 |
计算分子量 | 73 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC042101 |
基因名称 | DUSP16 |
Gene ID (NCBI) | 80824 |
RRID | AB_2878031 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BY84 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.
实验方案
Product Specific Protocols | |
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WB protocol for DUSP16 antibody 14237-1-AP | Download protocol |
IHC protocol for DUSP16 antibody 14237-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Discov NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving. | ||
Int Immunopharmacol Berberine derivative DCZ0358 induce oxidative damage by ROS-mediated JNK signaling in DLBCL cells | ||
J Mol Cell Cardiol Deficiency of smooth muscle cell ILF3 alleviates intimal hyperplasia via HMGB1 mRNA degradation-mediated regulation of the STAT3/DUSP16 axis | ||