• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16514-1-AP

Print datasheet

Synonyms

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

验证数据展示

  • WB analysis of mouse heart using 16514-1-AP

    WB analysis of mouse heart using 16514-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16514-1-AP (DUSP22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16514-1-AP

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 16514-1-AP

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16514-1-AP targets DUSP22 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DUSP22 fusion protein Ag9734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 22
Synonyms DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2095013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for DUSP22 antibody 16514-1-APDownload protocol
IHC protocol for DUSP22 antibody 16514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

FASEB J

DUSP22 suppresses prostate cancer proliferation by targeting the EGFR-AR axis.

Authors - Hsiu-Ping Lin
View Article
humanWB

Oncotarget

Molecular alterations and tumor suppressive function of the DUSP22 (Dual Specificity Phosphatase 22) gene in peripheral T-cell lymphoma subtypes.

Authors - Pierre Mélard
View Article