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Species Reactivity
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验证数据展示

  • WB analysis of mouse heart using 16514-1-AP

    WB analysis of mouse heart using 16514-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16514-1-AP (DUSP22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16514-1-AP

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 16514-1-AP

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 40x lens).

DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16514-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16514-1-AP targets DUSP22 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 DUSP22 fusion protein Ag9734 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 22
别名 DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
计算分子量 184 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC022847
基因名称 DUSP22
Gene ID (NCBI) 56940
RRIDAB_2095013
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for DUSP22 antibody 16514-1-APDownload protocol
IHC protocol for DUSP22 antibody 16514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

FASEB J

DUSP22 suppresses prostate cancer proliferation by targeting the EGFR-AR axis.

Authors - Hsiu-Ping Lin
View Article
humanWB

Oncotarget

Molecular alterations and tumor suppressive function of the DUSP22 (Dual Specificity Phosphatase 22) gene in peripheral T-cell lymphoma subtypes.

Authors - Pierre Mélard
View Article