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DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51005-2-AP

Print datasheet

Synonyms

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

验证数据展示

  • IHC staining of human lung cancer using 51005-2-AP

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 51005-2-AP

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 51005-2-AP

    WB analysis of HEK-293 using 51005-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51005-2-AP (DUSP22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51005-2-AP targets DUSP22 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 22
Synonyms DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2230783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP22 antibody 51005-2-APDownload protocol
IHC protocol for DUSP22 antibody 51005-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols