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验证数据展示

  • WB analysis using 68032-1-Ig

    WB analysis using 68032-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 68032-1-Ig

    IF Staining of NIH/3T3 using 68032-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DUSP22 antibody (68032-1-Ig, Clone: 3B8F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DUSP22 Monoclonal antibody

DUSP22 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68032-1-Ig

产品说明书

CloneNo. 3B8F1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Rabbit

应用

WB, IF/ICC, ELISA

别名

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68032-1-Ig targets DUSP22 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit
免疫原 DUSP22 fusion protein Ag9831 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dual specificity phosphatase 22
别名 DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
计算分子量 184 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC022847
基因名称 DUSP22
Gene ID (NCBI) 56940
RRIDAB_2918774
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for DUSP22 antibody 68032-1-IgDownload protocol
IF protocol for DUSP22 antibody 68032-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols