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验证数据展示

  • WB analysis using 15766-1-AP

    WB analysis using 15766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15766-1-AP (DUSP23 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 15766-1-AP

    IHC staining of mouse cerebellum using 15766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15766-1-AP (DUSP23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DUSP23 Polyclonal antibody

DUSP23 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DUSP23, DUSP25, LDP 3, LDP3, MOSP, RP11 190A12.1, VH1 like phosphatase Z, VHZ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15766-1-AP targets DUSP23 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DUSP23 fusion protein Ag8402 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 23
别名 DUSP23, DUSP25, LDP 3, LDP3, MOSP, RP11 190A12.1, VH1 like phosphatase Z, VHZ
计算分子量 17 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001140
基因名称 DUSP23
Gene ID (NCBI) 54935
RRIDAB_3085472
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP23 antibody 15766-1-APDownload protocol
IHC protocol for DUSP23 antibody 15766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols