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DUSP3 Polyclonal antibody

DUSP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28284-1-AP

Print datasheet

Synonyms

VHR, Vaccinia H1-related phosphatase, EC:3.1.3.16, dual specificity phosphatase 3

验证数据展示

  • WB analysis using 28284-1-AP

    WB analysis using 28284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28284-1-AP (DUSP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28284-1-AP

    WB analysis of HeLa using 28284-1-AP

    WB result of DUSP3 antibody (28284-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DUSP3 transfected HeLa cells.

  • IHC staining of human stomach cancer using 28284-1-AP

    IHC staining of human stomach cancer using 28284-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28284-1-AP (DUSP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28284-1-AP targets DUSP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DUSP3 fusion protein Ag28575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 3
Synonyms VHR, Vaccinia H1-related phosphatase, EC:3.1.3.16, dual specificity phosphatase 3
Calculated Molecular Weight 185 aa, 20 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC002682
Gene Symbol DUSP3
Gene ID (NCBI) 1845
RRIDAB_2918154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP3 antibody 28284-1-APDownload protocol
IHC protocol for DUSP3 antibody 28284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Immun Inflamm Dis

Dehydroandrographolide facilitates M2 macrophage polarization by downregulating DUSP3 to inhibit sepsis-associated acute kidney injury

Authors - Yanyan Shao
View Article