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验证数据展示

  • WB analysis using 30256-1-AP

    WB analysis using 30256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30256-1-AP (DUSP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 30256-1-AP

    IP experiment of A549 using 30256-1-AP

    IP result of anti-DUSP5 (IP:30256-1-AP, 4ug; Detection:30256-1-AP 1:500) with A549 cells lysate 1200 ug.

DUSP5 Polyclonal antibody

DUSP5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30256-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

VH3, HVH3, EC:3.1.3.16, DUSP, Dual specificity phosphatase 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HCT 116 cells
Positive IP detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30256-1-AP targets DUSP5 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 DUSP5 fusion protein Ag32997 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 5
别名 VH3, HVH3, EC:3.1.3.16, DUSP, Dual specificity phosphatase 5
计算分子量42kd
观测分子量 42 kDa
GenBank蛋白编号NM_004419
基因名称 DUSP5
Gene ID (NCBI) 1847
RRIDAB_3669707
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16690
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP5 (Dual specificity protein phosphatase 5) is also named as VH3. DUSP5, a member of DUSPs superfamily, is located in the nucleus and plays crucially regulatory roles in the signaling pathway transduction (PMID: 34169608). DUSP5 promotes the osteogenic differentiation of mesenchymal stromal cells (MSCs) by repressing SMAD1 signaling pathway in a SCP1/2‐dependent manner (PMID: 34169608). DUSP5, which specifically dephosphorylates extracellular signal-regulated kinase (ERK1/2), blocks pulmonary vascular smooth muscle cell proliferation (PMID: 34142888). DUSP5, an ERK1/2-specific endogenous phosphatase, was expressed at low levels in CRC (PMID: 36526622). DUSP5, a member of the DUSP subfamily, is known to regulate cellular inflammation (PMID: 33361528).

实验方案

Product Specific Protocols
WB protocol for DUSP5 antibody 30256-1-APDownload protocol
IP protocol for DUSP5 antibody 30256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols