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验证数据展示

  • WB analysis using 26718-1-AP

    WB analysis using 26718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26718-1-AP

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 26718-1-AP

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DUSP9 Polyclonal antibody

DUSP9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26718-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

MKP4, MKP 4, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, EC:3.1.3.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26718-1-AP targets DUSP9 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 DUSP9 fusion protein Ag25167 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 9
别名 MKP4, MKP 4, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, EC:3.1.3.16
计算分子量 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC060837
基因名称 DUSP9
Gene ID (NCBI) 1852
RRIDAB_2880612
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99956
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP9 antibody 26718-1-APDownload protocol
IHC protocol for DUSP9 antibody 26718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma

Authors - Xiang-Xu Wang
View Article
mouseWB

Sci Rep

Inhibition of miR-194-5p avoids DUSP9 downregulation thus limiting sepsis-induced cardiomyopathy

Authors - Jie Wang
  • KD Validated
View Article