验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse thymus tissue, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, MDA-MB-231 cells, NIH/3T3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12037-1-AP targets DVL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, xenopus |
| 免疫原 | DVL2 fusion protein Ag2666 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dishevelled, dsh homolog 2 (Drosophila) |
| 别名 | Dishevelled 2, Segment polarity protein dishevelled homolog DVL-2, DSH homolog 2, Dishevelled-2 |
| 计算分子量 | 736 aa, 79 kDa |
| 观测分子量 | 90-95 kDa |
| GenBank蛋白编号 | BC014844 |
| 基因名称 | DVL2 |
| Gene ID (NCBI) | 1856 |
| RRID | AB_2093330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14641 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DVL2 antibody 12037-1-AP | Download protocol |
| IHC protocol for DVL2 antibody 12037-1-AP | Download protocol |
| IF protocol for DVL2 antibody 12037-1-AP | Download protocol |
| IP protocol for DVL2 antibody 12037-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling. | ||
Biol Res Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model | ||
mBio Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner | ||
Cell Death Dis YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury. | ||
Development The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning. | ||
Front Cell Dev Biol Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition. |
