• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DYKDDDDK tag Recombinant antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Uni-rAbTM Recombinant Antibody for IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K14

Cat no : 80010-1-PBS

Print datasheet

Synonyms

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

验证数据展示

  • IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

The antibody 80010-1-RR is suggested for IP and IF.

产品信息

80010-1-PBS targets DYKDDDDK tag in IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity recombinant protein
Immunogen DYKDDDDK tag fusion protein Ag2329 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DYKDDDDK tag
Synonyms DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.