验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse kidney tissue, mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12001-1-AP targets Dymeclin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Dymeclin fusion protein Ag2600 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dymeclin |
| 别名 | DMC, DYM, Dymeclin, SMC |
| 计算分子量 | 669 aa, 76 kDa |
| 观测分子量 | 76-80 kDa |
| GenBank蛋白编号 | BC001252 |
| 基因名称 | Dymeclin |
| Gene ID (NCBI) | 54808 |
| RRID | AB_2246056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7RTS9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human Dymeclin is encoded by DYM gene, defects in which are the cause of Dyggve-Melchior-Clausen syndrome (DMC) and Smith-McCort dysplasia (SMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation, with cutaneous cells containing dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles [PubMed: 12491225]. Distinct with features of DMS, SMC is a rare osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest [PubMed: 19005420]. Dymeclin is a not a stably anchored transmembrane protein with a transmembrane domain, but it shuttles between Golgi and cytosol, which is necessary for correct organization of Golgi apparatus [PubMed: 18996921].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dymeclin antibody 12001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |