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DYNC1I1 Polyclonal antibody

DYNC1I1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13808-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

DNCIC1, DNCI1, DH IC-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inU-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy DYNC1I1 Ready-To-Use IHC Kit. DYNC1I1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13808-1-AP targets DYNC1I1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DYNC1I1 fusion protein Ag4810 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, cytoplasmic 1, intermediate chain 1
别名 DNCIC1, DNCI1, DH IC-1
计算分子量 645 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC022540
基因名称 DYNC1I1
Gene ID (NCBI) 1780
RRIDAB_2093492
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14576
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DYNC1I1 antibody 13808-1-APDownload protocol
IHC protocol for DYNC1I1 antibody 13808-1-APDownload protocol
IF protocol for DYNC1I1 antibody 13808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IHC

Oncogene

Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Authors - Heidi A Neubauer
  • KD Validated
humanWB

Front Mol Neurosci

MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons

Authors - Yongchao Mou
mouseWB

Neurobiol Dis

Pain sensitivity related to gamma oscillation of parvalbumin interneuron in primary somatosensory cortex in Dync1i1-/- mice

Authors - Zhongzhao Guo
  • KO Validated
mouseWB,IHC

Exp Gerontol

The potential benefits of PGC-1α in treating Alzheimer's disease are dependent on the integrity of the LLKYL L3 motif: Effect of regulating mitochondrial axonal transportation

Authors - Hou-Zhen Shi
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