DYNC1LI2 Polyclonal antibody
DYNC1LI2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue, human placenta tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DYNC1LI2 Ready-To-Use IHC Kit. DYNC1LI2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
18885-1-AP targets DYNC1LI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DYNC1LI2 fusion protein Ag13452 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dynein, cytoplasmic 1, light intermediate chain 2 |
| Synonyms | LIC53/55, LIC-2, LIC2, LIC 2, DNCLI2 |
| Calculated Molecular Weight | 492 aa, 54 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC025959 |
| Gene Symbol | DYNC1LI2 |
| Gene ID (NCBI) | 1783 |
| RRID | AB_10596910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytoplasmic dynein is a microtubule-associated motor protein. DYNC1LI2 (Cytoplasmic dynein 1 light intermediate chain 2), also known as LIC2, belongs to the dynein light intermediate chain family. DYNC1LI2 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It may play a role in binding dynein to membranous organelles or chromosomes. Northern blot analysis of rat tissues detected high expression of DYNC1LI2 in skeletal muscle, testis, heart, lung, and brain, and lower expression in liver, kidney, and spleen (PMID: 7738094).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC1LI2 antibody 18885-1-AP | Download protocol |
| IHC protocol for DYNC1LI2 antibody 18885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes. | ||
Cell Cycle Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. | ||
