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DYNLT3 Polyclonal antibody

DYNLT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11687-1-AP

Print datasheet

Synonyms

TCTEX1L, TCTE1XL, TCTE1L, T-complex-associated testis-expressed 1-like, RP3

验证数据展示

  • WB analysis of U-937 using 11687-1-AP

    WB analysis of U-937 using 11687-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 11687-1-AP (DYNLT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11687-1-AP

    IHC staining of human ovary tumor using 11687-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DYNLT3 antibody included
  • IHC staining of human ovary tumor using 11687-1-AP

    IHC staining of human ovary tumor using 11687-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DYNLT3 antibody included
  • IF Staining of HepG2 using 11687-1-AP

    IF Staining of HepG2 using 11687-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DYNLT3 antibody (11687-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inU-937 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DYNLT3 Ready-To-Use IHC Kit. DYNLT3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11687-1-AP targets DYNLT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen DYNLT3 fusion protein Ag2299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, light chain, Tctex-type 3
Synonyms TCTEX1L, TCTE1XL, TCTE1L, T-complex-associated testis-expressed 1-like, RP3
Calculated Molecular Weight 116 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC000968
Gene Symbol DYNLT3
Gene ID (NCBI) 6990
RRIDAB_2093777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51808
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dynein light chain Tctex-type 3 (DYNLT3) is a member of the cytoplasmic dynamic protein light chain Tctex family. DYNLT3 mainly plays a regulatory role in mitosis and meiosis, as well as chromosome binding. DYNLT3 is expressed in all cells and tissues. The calculated molecular weight of DYNLT3 is 13 kDa.

实验方案

Product Specific Protocols
WB protocol for DYNLT3 antibody 11687-1-APDownload protocol
IHC protocol for DYNLT3 antibody 11687-1-APDownload protocol
IF protocol for DYNLT3 antibody 11687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols