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验证数据展示

  • WB analysis using 18208-1-AP

    WB analysis using 18208-1-AP

    Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18208-1-AP (DYSFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 18208-1-AP

    IF Staining of THP-1 using 18208-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DYSFIP1 antibody (18208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DYSFIP1 Polyclonal antibody

DYSFIP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18208-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

Toonin, Protein phosphatase 1 regulatory subunit 27, PPP1R27, Dysferlin-interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat skeletal muscle tissue
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18208-1-AP targets DYSFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 DYSFIP1 fusion protein Ag12839 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dysferlin interacting protein 1
别名 Toonin, Protein phosphatase 1 regulatory subunit 27, PPP1R27, Dysferlin-interacting protein 1
计算分子量 154 aa, 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC093707
基因名称 DYSFIP1
Gene ID (NCBI) 116729
RRIDAB_3669295
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WC6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYSFIP1 is also known as PPP1R27. DYSFIP1 inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes. The molecular weight of DYSFIP1 is 17 kDa.

实验方案

Product Specific Protocols
WB protocol for DYSFIP1 antibody 18208-1-APDownload protocol
IF protocol for DYSFIP1 antibody 18208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols