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Di-Methyl-Histone H4 (Arg3) Recombinant antibody

Di-Methyl-Histone H4 (Arg3) Uni-rAbTM Recombinant Antibody for WB, ELISA, Dot Blot

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

1E21

Cat no : 82859-1-RR

Print datasheet

Synonyms

H4/a, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4, histone cluster 1, H4a, Histone H4

验证数据展示

  • Dot Blot experiment of peptide using 82859-1-RR

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 82859-1-RR

    WB analysis using 82859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 82859-1-RR

    WB analysis of mouse liver using 82859-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, ELISA, Dot Blot applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA, Dot Blot Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H4a
Synonyms H4/a, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4, histone cluster 1, H4a, Histone H4
Observed Molecular Weight13 kDa
GenBank Accession NumberBC069654
Gene Symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols