• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Dot Blot experiment of peptide using 84908-2-RR

    Dot Blot experiment of peptide using 84908-2-RR

    Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 84908-2-RR

    WB analysis using 84908-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84908-2-RR

    IHC staining of mouse testis using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse testis using 84908-2-RR

    IHC staining of mouse testis using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse liver using 84908-2-RR

    IHC staining of mouse liver using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse liver using 84908-2-RR

    IHC staining of mouse liver using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of human placenta using 84908-2-RR

    IHC staining of human placenta using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of human placenta using 84908-2-RR

    IHC staining of human placenta using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 84908-2-RR

    IF Staining of HeLa using 84908-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibody

Di/Tri-Methyl-Histone H3 (Lys4) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA
Cat No. 84908-2-RR

产品说明书

CloneNo. 241942D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Dot Blot, ELISA

别名

HIST1H3A, 241942D11, Histone-H3, HistoneH3, Histone H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, mouse liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84908-2-RR targets Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 241942D11, Histone-H3, HistoneH3, Histone H3
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
IHC protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
IF protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols