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E-cadherin Recombinant antibody, PBS Only

E-cadherin Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240159G8

Cat No : 80541-9-PBS

Print datasheet

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

验证数据展示

  • WB analysis using 80541-9-RR (same clone as 80541-9-PBS)

    WB analysis using 80541-9-RR (same clone as 80541-9-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80541-9-RR (same clone as 80541-9-PBS)

    IF Staining of MCF-7 using 80541-9-RR (same clone as 80541-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-9-RR, Clone: 240159G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

  • Affinity experiments of 80541-9-RR

    Affinity experiments of 80541-9-RR

    Biolayer interferometry (BLl) kinetic assays of 80541-9-RR against Human E-cadherin were performed. The affinity constant is 0.213 nM.

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

The immunogen of 80541-9-PBS is E-cadherin Fusion Protein expressed in E. coli.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human
Immunogen E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12830
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.