• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

    WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IF Staining of A431 using 83991-4-RR (same clone as 83991-4-PBS)

    IF Staining of A431 using 83991-4-RR (same clone as 83991-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with E-cadherin antibody included
  • Cytometric bead array standard curve of MP00187-6

    Cytometric bead array standard curve of MP00187-6

    Cytometric bead array standard curve of MP00187-6, E-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83991-4-PBS. Detection antibody: 83991-2-PBS. Standard: Eg1112. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83991-4-RR

    Affinity and Kinetic Characterization of 83991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83991-4-RR against Human E-cadherin were performed. The affinity constant is 2.78 nM.

E-cadherin Recombinant antibody, PBS Only (Capture)

E-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83991-4-PBS

产品说明书

CloneNo. 241098G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

83991-4-PBS targets E-cadherin as part of a matched antibody pair:

MP00187-6: 83991-4-PBS capture and 83991-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
计算分子量97 kDa
观测分子量135 kDa
GenBank蛋白编号NM_004360.5
基因名称 E-cadherin
Gene ID (NCBI) 999
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12830-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.