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验证数据展示

  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

E-selectin / CD62E Polyclonal antibody

E-selectin / CD62E Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30205-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IHC, IF-P, ELISA

别名

CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30205-1-AP targets E-selectin / CD62E in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原 E-selectin / CD62E fusion protein Ag31820 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selectin E
别名 CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E
计算分子量 610 aa, 67 kDa
GenBank蛋白编号BC142711
基因名称 CD62E
Gene ID (NCBI) 6401
RRIDAB_2935530
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16581
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

实验方案

Product Specific Protocols
IHC protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
IF protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols