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E-selectin / CD62E Polyclonal antibody

E-selectin / CD62E Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30205-1-AP

Print datasheet

Synonyms

CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E

验证数据展示

  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30205-1-AP targets E-selectin / CD62E in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen E-selectin / CD62E fusion protein Ag31820 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name selectin E
Synonyms CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E
Calculated Molecular Weight 610 aa, 67 kDa
GenBank Accession NumberBC142711
Gene Symbol CD62E
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

实验方案

Product Specific Protocols
IHC protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
IF protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols