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  • Featured Product
  • KD/KO Validated

E2F1 Monoclonal antibody, PBS Only

E2F1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D7G8

Cat no : 66515-1-PBS

Print datasheet

Synonyms

RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1

验证数据展示

  • WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

产品信息

66515-1-PBS targets E2F1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen E2F1 fusion protein Ag17363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name E2F transcription factor 1
Synonyms RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1
Calculated Molecular Weight 437 aa, 47 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC050369
Gene Symbol E2F1
Gene ID (NCBI) 1869
RRIDAB_2881878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01094
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.