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验证数据展示

  • WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

E2F1 Monoclonal antibody, PBS Only

E2F1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66515-1-PBS

产品说明书

CloneNo. 5D7G8

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66515-1-PBS targets E2F1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 E2F1 fusion protein Ag17363 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 E2F transcription factor 1
别名 RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1
计算分子量 437 aa, 47 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC050369
基因名称 E2F1
Gene ID (NCBI) 1869
RRIDAB_2881878
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01094
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.