验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, HuH-7 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10691-1-AP targets E2F6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | E2F6 fusion protein Ag1029 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | E2F transcription factor 6 |
| 别名 | E2F-6, E2F 6 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC008348 |
| 基因名称 | E2F6 |
| Gene ID (NCBI) | 1876 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75461 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
E2F6 is a member of the E2F family of transcription factors, which play important roles in cell cycle regulation and gene expression. E2F6 is highly expressed in many types of cancer and is associated with poor prognosis. It is involved in the regulation of cell proliferation and can promote tumor growth. E2F6 knockdown significantly reduces the proliferative ability of glioma cells (PMID: 38071912). Additionally, E2F6 is related to immune activation markers, tumor immune cell infiltration, and immune regulators, making it a potential biomarker for predicting the response to cancer immunotherapy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for E2F6 antibody 10691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |