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E2F6 Polyclonal antibody

E2F6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31464-1-AP

Print datasheet

Synonyms

E2F-6, E2F 6

验证数据展示

  • WB analysis using 31464-1-AP

    WB analysis using 31464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31464-1-AP (E2F6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 31464-1-AP

    IF Staining of Jurkat using 31464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using E2F6 antibody (31464-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31464-1-AP targets E2F6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen E2F6 fusion protein Ag35112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name E2F transcription factor 6
Synonyms E2F-6, E2F 6
Calculated Molecular Weight 32 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC008348
Gene Symbol E2F6
Gene ID (NCBI) 1876
RRIDAB_3669998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E2F6 is a member of the E2F family of TFs that control cell proliferation and cell fate. Cancer patients with high E2F6 expression in tumors, such as glioblastoma, breast cancer, and hepatocellular carcinoma, have poor prognosis.

实验方案

Product Specific Protocols
WB protocol for E2F6 antibody 31464-1-APDownload protocol
IF protocol for E2F6 antibody 31464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols