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E2F7 Recombinant antibody

E2F7 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230073C2

Cat no : 82887-1-RR

Print datasheet

Synonyms

Transcription factor E2F7, E2F-7, 230073C2

验证数据展示

  • IF Staining of HeLa using 82887-1-RR

    IF Staining of HeLa using 82887-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using E2F7 antibody (82887-1-RR, Clone: 230073C2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green).

  • FC experiment of HeLa using 82887-1-RR

    FC experiment of HeLa using 82887-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug E2F7 Recombinant antibody (82887-1-RR, Clone:230073C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82887-1-RR

    Affinity and Kinetic Characterization of 82887-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82887-1-RR against Human E2F7 were performed. The affinity constant is 31.3 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82887-1-RR targets E2F7 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen E2F7 fusion protein Ag21599 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name E2F transcription factor 7
Synonyms Transcription factor E2F7, E2F-7, 230073C2
Calculated Molecular Weight 911 aa, 100 kDa
GenBank Accession NumberBC136366
Gene Symbol E2F7
Gene ID (NCBI) 144455
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96AV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )

实验方案

Product Specific Protocols
IF protocol for E2F7 antibody 82887-1-RRDownload protocol
FC protocol for E2F7 antibody 82887-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols